Enzyme Kinetics Question

Ray Deems rad at chem.ucsd.edu
Fri Apr 22 11:15:41 EST 1994

In article <9404111941.AA22231 at unixg.ubc.ca> flo at UNIXG.UBC.CA writes:
>If an enzyme has such an incredibly low activity on a substrate that you 
>need to use equal molar amouts of enzyme to substrate, how does this 
>affect the kinetics ?   ie can you still use the Michealis-Menten type 
>equations even though the enzyme concentration is no longer much smaller 
>than the substrate concentration?????

It is still an enzyme, whether it is catalyzing the reaction
depends upon the rates in the presence and absence of the 
enzyme.  However, the standard enzyme kinetic equations definitely
do not apply.  One of the basic assumptions in deriving them
is that the free substrate concentration remains constant.
This would not be true in this case.  You would have to derive
new equations taking this into account

Ray Deems

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