Phage display of Small Polypeptides

[flo at UNIXG.UBC.CA]

Netters :

There is lots of literature where random short peptides are cloned and
pannned using the phage display method; my question is which display phage
vector/host system is most reliable and suitable for successfully
displaying 1 copy per phage of a 100 aa domain of a protein (i.e. a small
self-folding polypeptide) ? Are these systems stable, or do they delete /
mutate at high frequency ? What are the most relevant potential
difficulties ? Can anyone point me to pertinent references ?

Thanks,

Roger Graham
Roger Graham
Dept. of Microbiology
University of British Columbia
Vancouver B.C. Canada  V6T 1W5
flo at unixg.ubc.ca