Sequencing gel

Steven Coon at
Fri Apr 22 10:08:10 EST 1994

> > > I have had that problem when I used plates the first time after
> > > siliconizing them with dichlorodimethylsilane.  The gel used to crawl out
> > > into the top tank.  You can avoid this by washing the plates with a
> > > slightly abrasive powder detergent after you siliconize them.  That always
> > > worked for me.
> > > 
> > 
> > In our lab, we found that Rain-X (found in auto shops) is a great
> > substitute for silicon, and less toxic.  We avoid the "crawling" phenomenon
> > by only Rain-Xing the short plate, so that the gel sticks to the long
> > plate.
> I also use Rain-ex now but still like to siliconize both plates.  I don't
> have a crawling problem as long as I was the plates with comet before I use
> them.

no matter what the gel must stick to one plate more than the other.

Steve Coon
sdcoon at
University of Michgan Medical Center

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