Wanted: lambda EMBL3/EMBL4 sequences

Francis Ouellette francis at borduas.nlm.nih.gov
Sat Apr 23 16:15:58 EST 1994


Klaus (knoblauch at mpimg-berlin-dahlem.mpg.de) writes:

: I wonder, whether any of you has ever obtained/assembled/got from wherever
: the DNA-Sequence of the cloning Vector lambda EMBL3 or lambda EMBL4.

: PS: I've already tried searches in GenBank and EMBL and VEC and gophered 
:    to gopher.gdb.org but ...  :-{

Dear Klaus, 

the EMBL3 righ and left arms are out there, and there are
different ways of getting them.  You do have to do a little
investigating.  But the accession numbers you are looking for are:


U02425: Cloning vector lambda EMBL3, left arm.
U02453: Cloning vector lambda EMBL3, right arm.


1) by gopher:

gopher to  ftp.bio.indiana.edu

and look at the genbank directory, searching with "embl3 and vector" 
I obtained this list:

----------------------------------------------------------------------

                          List of matches to your query
 Database:     GenBank biosequence data
 Server:       ftp.bio.indiana.edu
 Search words: "embl3 and vector"

(1) X06780 Phage lambda vector EMBL3 polylinker (partial)
(2) X58667 Bacteriophage lambda EMBL3 right arm DNA with git gene, partial cds
(3) X58668 Bacteriophage lambda EMBL3 left arm DNA with int gene, partial cds
(4) M96073 Arabidopsis thaliana phosphoribosylanthranilate transferase (PAT1)
(5) L04147 Human neurofilament light chain (NEFL) gene, promoter region.
(6) U02425 Cloning vector lambda EMBL3, left arm.
(7) U02426 Cloning vector lambda EMBL3 SP6/T7, left arm.
(8) U02427 Cloning vector lambda EMBL3 SP6/T7, right arm.
(9) U02453 Cloning vector lambda EMBL3, right arm.

----------------------------------------------------------------------

This was obtained a gopher+ client which has some very nice features.

2) Another way is to use the GenBank retrieve server.  This only
   requires an E-mail package ... and allows for searching within
   specific fields (which can be very useful in some cases).

So if you send help to the retrieve server, you will get info on how
to format your querry.  The address is:

retrieve at ncbi.nlm.nih

if you send this query:

datalib genbank
titles
begin
embl3 [src] and vector [src]

you get this back:


=============================================================================
To Obtain Help Documentation:  send e-mail to 'retrieve at ncbi.nlm.nih.gov' 
		 with the word 'help' in the body of the mail message.
=============================================================================


   1) [U02426]    Cloning vector lambda EMBL3 SP6/T7, left arm.
   2) [U02425]    Cloning vector lambda EMBL3, left arm.
   3) [U02427]    Cloning vector lambda EMBL3 SP6/T7, right arm.
   4) [U02453]    Cloning vector lambda EMBL3, right arm.
   5) [HUMNFL]    Human neurofilament light chain (NEFL) gene, promoter region.


To retrieve the two files you want, send this message:

datalib genbank
begin
U02425 [acc]
U02453 [acc]

and you will get the two files you want in the return mail (usually
less than a minute, bit will vary with connection you have,
geographical location, time of day and so on)

3) Another way is to use Entrez, a DNA/protein/bibliographical tool
   which allows you to get at this information in many ways.  This 
   program works by accessing the information on a CD-Rom on your PC
   Mac, Unix or VMS machine, or a local server.  There is also Nentrez 
   (network entrez) which works over the Internet to access the
   database at NCBI.  If you want more info about this software, and
   how to obtain it, send a message to: 
   
   info at ncbi.nlm.nih.gov

   The people who answer that mail will be more than happy to help you
   to get started with Entrez (what ever flavor).


regards,

francis


--
| B.F. Francis Ouellette  
|
| francis at ncbi.nlm.nih.gov   



More information about the Methods mailing list