PLASMIDS STUCK ON NITROCELLULOSE

Shahram Mori smori at nmsu.edu
Sat Apr 23 21:34:39 EST 1994


Philip OBrien (obrien at CSUVAX1.MURDOCH.EDU.AU) wrote:
: I received a sample of a plasmid in the form of a drop of DNA on 
: nitrocellulose.  I want to transform this into a bacterial strain.  What 
: is the best way to desorb the plasmid DNA from the nitrocellulose so that 
: it can be transformed?

: P. O'Brien
: Perth Western Australia
: obrien at csuvax1.murdoch.edu.au

Dear philip,
I had always thought that the binding of DNA to Nitrocell.filters was
irreversible. However as you know with time nitrocellulose loses its form and
DNA is lost. I wonder if a long term incubation with TE would trap the
DNA. This is just a suggestion. You may wanna try this with an
experimental plasmid just to check and see.
Cheers.
Shahram Mori
Program in Molecular Biology
Dept. of Chemistry Box 3C
NMSU   Las Cruces NM
88003

  _/\_
 _\  /_
 \_  _/
   ||



More information about the Methods mailing list