radioactive 5'label.mRNA?

[Martin Leach]

In article <2pgmva$i4b at wega.rz.uni-ulm.de>, SCHU at rzmain.rz.uni-ulm.de (Schu
Bernhard) wrote:

> I have problems with the radioactive 5'-phosphorylation of 
> a large (1500 bases) mRNA with PNK.
> Who has  any experiences or suggestions for solving that 
> problem ?
> 
> Thanks :-)
> Bernd
> __________________________________________________________
> 
> Bernhard Schu		Tel: +49/731/5022322
> Sektion Polymere	FAX: +49/731/5023159
> Universitaet Ulm


Could the cap on the 5'end of the mRNA be causing the problem. If so,
remove the cap and try again.

Or add radiation at the 3' end using Terminal deoxynucleotide transferase.

Martin


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.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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