Inverse PCR

Greg Denomme denomme at FHS.CSU.MCMASTER.CA
Mon Apr 25 11:47:58 EST 1994



On Sat, 23 Apr 1994, Joshua Daniels wrote:

> Hello-
> 
> I am interested in obtaining a protocol for inverse PCR.  This technique 
> allows you to amplify a restriction fragment cut by one enzyme.  If the 
> fragment of interest has a known marker (You do a Southern first ), you ligate 
> the ends of the restriction fragment, thereby circularizing it and you can use 
> 2 primers within the known sequence to do PCR.
> 
> Thanks much,
> 
> Joshua Daniels
> **************************************************************************************************************************
> Joshua Daniels                                                                UW Madison                                                
> jbdaniel at facstaff.wisc.edu                                                Med. School
> 608-257-2335                                                                   Dept. of Anatomy 608-262-3327
> **************************************************************************************************************************
> 

The reference is:

Triglia T, Peterson MG and Kemp DJ. (1988). A procedure for in vitro
amplification of DNA segments that lie outside the boundaries of known
sequences. NAR 16:8186.

Cheers,
Greg Denomme, PhD
denomme at fhs.csu.mcmaster.ca






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