Nested-deletions paradox

[Dave Boyd]

I think the critical factor to avoid a wide range of deletion sizes at each
timepoint is to start with a plasmid prep that is as much supercoiled as
possible i.e. minimum nicking. The best would be a CsCl prep but I hate
taking the time so I always used Magic Miniprep DNA (what will I use now?).
 I found you always get unexpected-sized plasmids at each timepoint but I
still only had to screen 3-5 transformants per timepoint to get at least 1
of the expected size.

Dave Boyd
dboyd at ccu.umanitoba.ca
Dept of Oral Biology
University of Manitoba