Nested-deletions paradox

Dave Boyd dboyd at ccu.umanitoba.ca
Mon Apr 25 10:25:31 EST 1994


I think the critical factor to avoid a wide range of deletion sizes at each
timepoint is to start with a plasmid prep that is as much supercoiled as
possible i.e. minimum nicking. The best would be a CsCl prep but I hate
taking the time so I always used Magic Miniprep DNA (what will I use now?).
 I found you always get unexpected-sized plasmids at each timepoint but I
still only had to screen 3-5 transformants per timepoint to get at least 1
of the expected size.

Dave Boyd
dboyd at ccu.umanitoba.ca
Dept of Oral Biology
University of Manitoba



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