Thermal Cyclers

[Dr. M.D. Jones]

Hi netters

apologies if this is repeat question

I do a fair amount of cycle sequencing for an ABI 373A instrument
and I am getting tired of using oil to cover the 96-well microtitre
plate for DYE-Primer reactions.  The use of the strip tubes (PEC or
Robbins) becomes expensive.

So the questions are

1. has anybody a system for sealing 96-well microtitre plates for use
with pelletier cyclers ?

2. How well do the thermal AIR cyclers perform for cycle-sequencing ?

Mick Jones
Department of Virology
RPMS, London
W12 0NN, UK
Tel: 44 81 740 3328
FAX: 44 81 743 8331
email: mjones at rpms.ac.uk