leach at mbcrr.harvard.edu
Tue Apr 26 21:16:10 EST 1994
In article <-260494103555 at dent-226-97.dentistry.umich.edu>, @umich.edu
(Steven Coon) wrote:
> Hi netters,
> I am about to reverse transcribe the RNA and was considering using
> Clontech's 1st strand cDNA synthesis kit designed for this purpose. I would
> like any feedback on the kit or suggestions for alternatives.
Clonetech is expensive. Their RNA is full of DNA and SLIC should be renamed
BTW if doing RT-PCR - DNAse treat your RNA before RTing it. Otherwise
unless u r pcring over an intron (in the DNA) u won't know whether the
product is real or due to DNA contam.
Also i think their is a difference between MuLV and AMV RT in that one of
these chops back at the 3'end....not sure which...
..... Martin Leach Email:leach at mbcrr.harvard.edu
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