Strange agarose gel ethidium bromide staining fading

Bill Jack jack at neb.com
Tue Apr 26 08:55:10 EST 1994


In article <199404142312.JAA22037 at sol.ccs.deakin.edu.au>,
huangbx at DEAKIN.EDU.AU wrote:

> Hello Netters,
> 
> I am encountered an extraordinary strange phenomenon agarose gel ethidium
> bromide staining fading.
> 
> Does anyone have this sort of experience? Could you kindly give me some
> advice? Or any comments will be very appreciated!
> 
> Thank you very much for your time.
> 
> Bixing Huang
> huangbx at deakin.edu.au 

An article that may be relevant is:
"Comparison of Various Ultraviolet Sources for Fluorescent Detection of
Ethidium Bromide-DNA Complexes in Polyacrylamide Gels"
C.F. Brunk and L. Simpson
Analytical Biochemistry 82:455-462 (1977)

They surveyed 254, 300, and 366 nm light sources, and looked at
photodamage, photonicking, photodimerization, and photobleaching. They also
evaluated the amount of fluorescence generated by the different
wavelengths. 254 nm "produced a great deal of photodamage,..., and
photobleaching."

Have you changed light sources recently?

Bill Jack
New England Biolabs
jack at neb.com



More information about the Methods mailing list