Making fusion proteins with protein kinase sites for 32P labelling

T. S. Pillay tpillay at ucsd.edu
Tue Apr 26 23:36:30 EST 1994


Subject: Making fusion proteins with protein kinase sites for 32P
labelling
From: Simon Kidd, kidd at rockyj.rockefeller.edu
Date: 26 Apr 94 17:34:39 GMT
In article <1994Apr26.133440.24294 at rockyd.rockefeller.edu> Simon Kidd,
kidd at rockyj.rockefeller.edu writes:
>Hi
>I wonder if anyone knows about  a system in which the protein of
interest is
>fused to a protein kinase site enabling it to be labelled to high
specific
>activity with 32P? Does such a vector exist? If not what would be the
best
>kinase to use (ie commercially available and infrequent sites)?
>Thanks in advance

Pharmacia sells a version of the ubiquitous pGex which has  a
cAMPdependent kinase consensus phos. site.



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