PCR: hot start

Hank Seifert h-seifert at nwu.edu
Wed Apr 27 11:20:30 EST 1994


[stuff deleted]

>> > On Wed, 13 Apr 1994, Mgr.Jiri Drabek wrote:
>> >
>> >> Hello, netters!
>> >> Please, does anybody know alternative (cheap, home made) for PERKIN-ELMER
>> >> AmpliWax PCR gem (used for hot start)?
>> >> Any answer will be appreciated.

We and others have published that ordinary Parafin Wax works 
fine for hotstart.  {Wainwright & Seifert, 1993.  BioTechniques 
14:34-36, and a Trends in Genetics paper in 1993}. In fact, I think 
AmpliWax is just parafin.



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