How can we directly sequence PCR-amplified DNA from agarose.

Wasun Chantratita asmsi002 at CMU.CHIANGMAI.AC.TH
Wed Apr 27 09:44:12 EST 1994


Dear Netters
	I am now trying to directly sequence PCR-amplified DNAs by cycle 
sequencing. I was told that excess nucleotide and amplified primers can 
interfere with the sequencing reactions. Thus,  PCR-amplified DNAs must 
be purified by either a glass matrix or isopropanol precipitation. 
However, would it be possible that we can cut a DNA band from perhaps low 
MP agarose and subject to cycle sequencing directly. If yes, would you 
please provide me that protocol or reference(s).

	Many thanks in advance
        Wasun
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Wasun Chantratita, Ph.D.                        Phone:053-221122 Ext 5086,5068
Department of Clinical Microbiology             Fax:  053-221890 
Faculty of Associated Medical Sciences       Email:asmsi002 at cmu.chiangmai.ac.th
Chiang Mai University
Chiang Mai 50200
Thailand
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