Using glycerol to enhance PCR amplification.

Wasun Chantratita asmsi002 at CMU.CHIANGMAI.AC.TH
Wed Apr 27 09:30:46 EST 1994


	A few days ago, there was a request from someone who would like to
know the real effect of glycerol in enhancing pcr amplification. I may
suggest him to read "Amplification", Sep 1990 Issue 5 published by the
Perkin-Elmer Corporation. In brief, you can add 5-20% of glycerol into
your PCR reaction mixture. It will effectively increase the amount of your
amplified product from G/C-rich DNA template. I always add 10% of glycerol
in order to get 950 bp amplicon from G/C-rich Herpes simplex virus genome
(template). I guest that glycerol might help PCR reaction mixture to
accumulate enough heat to denature G/C-rich double stranded DNA template 
and do also stabilize Taq DNA Pol at the same time. Sorry in advance if I 
am wrong.

Wasun
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Wasun Chantratita, Ph.D.                        Phone:053-221122 Ext 5086,5068
Department of Clinical Microbiology             Fax:  053-221890 
Faculty of Associated Medical Sciences       Email:asmsi002 at cmu.chiangmai.ac.th
Chiang Mai University
Chiang Mai 50200
Thailand
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