Martin Leach leach at
Wed Apr 27 22:03:07 EST 1994

Dear netters,

i feel so demanding lately.

Ok, would love any of the following info.

1. Good refs for run-off assays (promoter construct u add nuclear extract
to and make transcripts).
2. Names/phone no.s of anyone in the boston area that routinely perform

3. Any good run-off kits out there (pros/cons etc...)

4. Any reviews on the pros/cons of run-offs vs. run-on assays.

5. Preparation of nuclear extracts for run-off assays.

6. ANything else (preferably someone that will perform them for free!!)

I think u can tell by now i am very interested in run-off assays. Any and
all info will be warmly received.

I would prefer email responses (although i encourage responses to be posted
for the masses also).




.....          Martin Leach                Email:leach at 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER 
p.s. try BioMOO (virtual biology on the internet - telnet 8888)

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