Vaccinia virus/Expression

Ashu Sharma orbashu at
Thu Apr 28 17:02:00 EST 1994

selection of recombinant virus I am using TK- cell line. My problem is that 
although I do see lot of blue plaques after x-gal staining none of them is 
positive for my gene.  I will graetly appreciate if somebody out there who is 
using the similar system could give me hints or good protocol. Thanks in advance
Ashu Sharma

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