long PCR's reference (fwd)

Robert Preston rapr at MED.PITT.EDU
Thu Apr 28 10:35:29 EST 1994


> From: jr at dna.bio.warwick.ac.uk
>           Does anyone know of a reference that has successfully amplified a 
> large PCR fragment (approximately 8kb). I think one was posted a while ago.
> Thanks in advance
> Jaz
One ref is W.M.Barnes Proc. Natl Acad. Sci USA 91:2216-2220 1994
a pretty exciting result (35 kb fragment amplified) although 10 exp 6 to
10 exp 8 copies of the lambda phage target was used.

Rob
rapr at med.pitt.edu 
P.S. Also see news section of Science a few weeks ago for related info.





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