5'-RACE: Kits vs "homemade" reagents

John Watson ajwatson at romeo.caltech.edu
Thu Apr 28 11:23:06 EST 1994


In article <leach-260494212840 at ppp-8-28.bu.edu>, leach at mbcrr.harvard.edu
(Martin Leach) wrote:
> 
> In article <2pjmss$gke at dartvax.dartmouth.edu>,
> Anitra.S.Auster at dartmouth.edu (Anitra S. Auster) wrote:
> 
> > We are trying to sample opinion on the relative merits (or demerits) of
> > the GIBCO/BRL vs the Clontech 5'RACE kits.   Another option is
> > obviously to just make up our own reagents.  Any general advice about
> > 5'-RACE also appreciated. Please respond to
> > anitra.auster at dartmouth.edu.
> > 
> >   Many thanks in advance.
> 
> Do either of these use Tobacco acid pyrophosphatase to remove caps....
> 
> Martin
> <stuff deleted.

Neither kit removes capped ends.

John



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