John Watson ajwatson at romeo.caltech.edu
Thu Apr 28 11:20:26 EST 1994

In article <-260494103555 at dent-226-97.dentistry.umich.edu>, @umich.edu
(Steven Coon) wrote:
> Hi netters,
>     I am about to reverse transcribe the RNA and was considering using
> Clontech's 1st strand cDNA synthesis kit designed for this purpose. I would
> like any feedback on the kit or suggestions for alternatives.
> thanx
> Steve Coon
> sdcoon at umich.edu
> University of Michigan Medical Center

I have used Clontech 1st strand kits as well as BRl kits and home-made
mixes.  They all seem to work just fine.  I kind of like the Clontech kit
over the BRL kit.

I have not found thast DNasing the sample is necessary, but we were PCRing
a large intron and so would not expect DNA contamination.  I guess it all
down to how much you know about your expected product.  The -RT control,
mentioned in a previous response is a good idea, IMHO.


More information about the Methods mailing list