Fusion proteins

David L. Haviland, Ph.D. HAVILAND at KIDS.WUSTL.EDU
Thu Apr 28 21:22:42 EST 1994


Hi.

I have a question for those using any of the fusion protein expression 
systems such as GST and the like.  We are interested in setting this 
technology up in our lab.  Our goal is not only to make the fusion peptides 
as a source of purified peptide by cleaving off the GST but also for use in 
immunization, and thatt's where my question lies.

Ig GST is kept on the fusion peptide by eluting it off the column, can the 
GST serve as the carrier in immunizations, circumventing the necessity of 
coupling the peptide to a more traditional carrier like KLH?

I know this can be done using the pRIT-2T vector from Pharmacia, but I have 
read in the literature (and heard from the director of the local hybridoma 
facility) that immune response directed against fusion protiens carrying 
protein-A often have the immune response biased toward the Protein-A and 
not the peptide.  Is this phenomena been observed using GST or MBP based 
vectors?

Thanks in advance,
David 

(also crossposted (I hope) on the bionet.immunology sub)

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