Northern Blot Background

Scott Bearden swb at seqanal.mi.uky.edu
Fri Apr 29 16:31:22 EST 1994


Does anyone have information about eliminating non-specific hybridization to
rRNA on northern blots when using plasmid-derived [32]P-labeled probes?  The
blot was blocked with denatured salmon sperm DNA prior to hybridization with a
plasmid probe having a GC content of approximately 56%.

Scott Bearden
swb at seqanal.mi.uky.edu



More information about the Methods mailing list