Northern Blot Background

Scott Bearden swb at
Fri Apr 29 16:31:22 EST 1994

Does anyone have information about eliminating non-specific hybridization to
rRNA on northern blots when using plasmid-derived [32]P-labeled probes?  The
blot was blocked with denatured salmon sperm DNA prior to hybridization with a
plasmid probe having a GC content of approximately 56%.

Scott Bearden
swb at

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