Glowing Blue DNA Gels (fwd)

Robert Preston rapr at MED.PITT.EDU
Fri Apr 29 09:46:29 EST 1994


> >> What the . . . ?
> >> 
> >> The last 2 times I ran agarose gels (TAE buffer, stained in dilute ethidium
> >> bromide/water), I put them on the UV box and they glowed 
> >> 
> >> 	BRIGHT BLUE ALL OVER.
> >> 
> >> This made it very difficult to see the orange EtBr/DNA bands. 
> >> 
Just paste some of that orange Wratten filter used for photography on your
safety glasses: it blocks the blue fluorescence but lets orange DNA/EtBr
come through.  Possibly you're dealing with a 10X too-high error on the
stock EtBr concentration?

Rob
rapr at med.pitt.edu




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