Stopcodons in phage fusevectors ?
kjaer at biobase.aau.dk
Sat Apr 30 08:16:03 EST 1994
Recently, I made a selection using a phage random 15mer-peptide library
kindly provided by Smith. An increase in titer was observed after several
rounds of biopanning so I started sequencing, but to my big surprise
UAA codons appeared. So my questions is: Is the E.coli strain K91kan
suppressing the UAA codons or are there any other explanation to how viable
phages can be made when a stopcodon is present in the insert ?
Thanks a lot
More information about the Methods