Stopcodons in phage fusevectors ?

Svend Kjaer kjaer at biobase.aau.dk
Sat Apr 30 08:16:03 EST 1994


 Recently, I made a selection using a phage random 15mer-peptide library 
kindly provided by Smith. An increase in titer was observed after several
rounds of biopanning so I started sequencing, but to my big surprise 
UAA codons appeared. So my questions is: Is the E.coli strain K91kan
suppressing the UAA codons or are there any other explanation to how viable
phages can be made when a stopcodon is present in the insert ?

Thanks a lot
 
Svend



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