Methods April 1994 Archives by date

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Starting: Fri Apr 1 00:54:51 EST 1994
Ending: Sat Apr 30 18:36:48 EST 1994
Messages: 882

5' biotin labeling of oligos Steve Rodems
Gel Shift Probe Steve Rodems
long range PCR!!! Seeking papers!!! Fabrice Glibert
Kanamycin or G418 for plant transformation? Hanson Lab
BioRad's Prep-A-Gene, try it, you'll like it Bruce Roe
Gel Shift Probe Martin Leach
Cleaning seq. plates with "Bon Ami"?? Sue Jeffrey
Protein purification? W. Chen
Long PCR by FTP Wayne M. Barnes, Ph.D.
Cleaning seq. plates with "Bon Ami"?? Shiao Y. Wang
5' biotin labeling of oligos Shiao Y. Wang
PCR for 6-7 kb Shiao Y. Wang
Cleaning seq. plates with "Bon Ami"?? Dan Diaz
Do you know where I can find E. coli GC6?? Hank Seifert
Software for the prediction of coiled coils in proteins Nicholas J. Morris
RNA protection assays Marianna Max
Two-promoter expression vector Mendell Rimer
pBDH001 Tracey Zacharias
Site directed mutagenesis Abiye Iyo
RNA Gel rphjr at nwu.edu
Availability of testicular and epididymal cDNA libraries Michael Holland
Actin probes in Northerns Kelly Tetro
Do you have to sequence both strands? Zhongguo Xiong
Do you have to sequence both strands? John Gosink
Storage of T4 phage I-shiou Ng
Two-promoter expression vector Brian Foley
Summary for "Non-specificity for Primer Extension?" LIU at QUCDN.QueensU.CA
need source for Xca I restriction endonuclease Jack S. Benner
Sequencing gel apparatus suggestions Sean David Moore
Fucosyl Transferase WANTED Steven Striepeke
Do you have to sequence both strands? TOM PARSONS
Do you have to sequence both strands? Thomas H. Rude
(none) Liam E Good
MACS cell purification Gene Holowachuk
Bio-Rad wzhao at mbcr.bcm.tmc.edu
Superpolylinker vectors-source? ed beaty
RAIN-X on Sequence Gels? Kirk A. Bartholomew
Do you have to sequence both strands? Martin Kennedy
Gel Shift Probe gc
Protein purification? gc
(none) krishnan at BORCIM.WUSTL.EDU
Do you have to sequence both strands? Michael Holland
2-ME effect on lymphocyte proliferation assay Wasun Chantratita
Do you have to sequence both strands? dpp at icbr.ifas.ufl.edu
ABI Sequencer-Dyed terminat Condie Carmack
Summary for "Non-specificity for Primer Extension?" Steve Rodems
Superpolylinker vectors-source? Alberto I Roca
Gel Shift Probe h9290184 at hkucc.hku.hk
Missense translation? Ann Lund
Syntex address Damak, Sami
Isolating YAC DNA Koen Dechering
Summary for "Non-specificity for Primer Extension?" Martin Leach
Immunoscreen needed Mr. Steven Swift; BIOL; GRAD
Actin probes in Northerns Pranee Leechanachai
Long PCR / address of Ab peptides(St Louis) Martin Leach
(none) (formaline profused tissue RNA) Alexandra
Do you have to sequence both strands? Brian Foley
Summary for "Non-specificity for Primer Extension?" Dan Diaz
methods for cell sorting required Ms S.P. Moyes
Antibodies to MARCKS (PKC substrate) Yves Durocher
Where is Kevin Jones Hank Seifert
Prof CC Wang's Address sought A. E. Souza
On-Line RFAs, duplic. to virol,methods Marion Freistadt
dextran sulfate-- a cheaper source?? Michael Man
E-mail Address of SynapSys Corp. riyer at RASCAL.MED.HARVARD.EDU
Re; address of ab Peptides FrohlichM
question regarding RACE kits Feng Jin
Solubilization of membrane protein ! G. Jawahar Swaminathan
DNA isolation from blood ez033285 at hamlet.ucdavis.edu
Northern Problem Adrian Molenaar
Sequencing Gel adheres to both plates Wasun Chantratita
Wizard/Magic Preps Scott J. Diede
luciferase as in vitro translation control Hinayana Bawagan
DynaZyme DNA Polymerase Kit Zainul Zain
125-I in liquid scintillation counter E.L. Newman Surgery ext ninewells 3174
Percoll Gradients Denise Carpenter
anti beta gal antibody Denise Carpenter
question regarding RACE kits Matthias Zeiner
Library Amplification craig.rowlands at csb.ki.se
Rat cDNA library Bengt Widegren
Wizard/Magic Preps Martin Leach
Drying of PAGE gels - Cellophane Martin Leach
Drying of PAGE gels - Cellophane oesch at hifo.unizh.ch
Sequencing gel apparatus suggestions Sean David Moore
Used equipments Janis Eisaks
Sequencing Gel adheres to both plates Michael van Waes
What is going on? Janis Eisaks
in situ/immuno combo KGEISS%RAVEN at EAGLE.AAMRL.WPAFB.AF.MIL
RAIN-X on sequencing gels russos at HAL.HAHNEMANN.EDU
Sequencing gel combs (leakage) Paul N Hengen
B Cell Specific Antibody Nancy McNelly
cRNA wzhao at mbcr.bcm.tmc.edu
cDNA libraries amitchell at mbcl.rutgers.edu
A Hypothetical question!!! Shahram Mori
Wizard/Magic Preps J. David Spafford
Flu-HA tag Richard N. Day
PCR from genomic DNA krishnan at BORCIM.WUSTL.EDU
Wizard/Magic Preps James S. Sutcliffe
RAIN-X on Sequence Gels? Steven Coon
dextran sulfate-- a cheaper source?? Steven Coon
Sequencing Gel adheres to both plates Steven Coon
Merlin (was Re: Wizard/Magic Preps) Bob DeLisio
SURVEY: How do you get rid of ethidium bromide waste Kieron Des
CD-3 Antibody? Scott Selby Med Heme
Looking for T7 polymerase gene U10784 at uicvm.uic.edu
stratagene pCR-script--- good or bad? john hess
threonic acid John Deutsch
RACE kit Martyn White
LIBRARY PLATING: no lysis of bacterial lawn Kevin O Hwang
luciferase as in vitro translation control Dave Bates
stratagene pCR-script--- good or bad? Klaus.Matthaei at ANU.EDU.AU
Oligo. programs Sarven
Query on the Barnes paper/ Long range PCR Bentley Atchison
Antibody against chloroplast RNA polymerase beta" subunit? Zengjian Chen
Oligo. programs Condie Carmack
RAIN-X on Sequence Gels? Dan Diaz
Oligo. programs John Brunstein
A Hypothetical question!!! Martin Leach
DynaZyme DNA Polymerase Kit Timo Hiltunen
question regarding RACE kits Timo Hiltunen
??curr.prot. in mol.biol.??-ivo w. Ivo Wiesner
Reverse Transcriptase moulliard at mbla.ucl.ac.be
Do you have to YES!! Tony Hodge
Protease inhibitors schren13 at uctvax.uct.ac.za
Construct with human tyrosinase promoter Eivind Hovig
methods for cell sorting required Andy
MACS cell purification Andy
methods for cell sorting required Martin Leach
in situ/immuno combo David M. Berman
Staining PVDF (Immobilon) David M. Berman
LIBRARY PLATING: no lysis of bacterial lawn Angela Jones
e-mail address? jac33 at sm.ic.ac.uk
long pcrs brett at BORCIM.WUSTL.EDU
RAIN-X on sequencing gels the End
Staining PVDF (Immobilon) Ding Ming
'Tandem' Primers for PCR Mutagenesis Shaun D. Black
Oligo. programs John Brunstein
protein expression, PET system Geethanjali Akula
Protein similarity via hydropathy plots? Michael Coyne
"tandem" primers for pcr mutagenesis brett at BORCIM.WUSTL.EDU
'Tandem' Primers for PCR Mutagenesis FJVAND00 at ukcc.uky.edu
Codon Usage Tables Warren Gallin
PCR from genomic DNA Mr. Steven Swift; BIOL; GRAD
'Tandem' Primers for PCR Mutagenesis Basavaraju Shankarappa
Looking for T7 polymerase gene Virginia Dress
Brookehaven protein structure database decker at lenti.med.umn.edu
IF for neutrophils Seth Corey
More 5'-RACE kits Brett Levay-Young
PDB Database Shaun D. Black
Protein similarity via hydropathy plots? Shaun D. Black
RNA from teeth? Steven Coon
'Tandem' Primers for PCR Mutagenesis Hong Dang
Looking for T7 polymerase gene Zophonias Oddur Jonsson
DynaZyme DNA Polymerase Kit Zophonias Oddur Jonsson
Reverse Transcriptase Michael van Waes
Dephosphorylating pACYM1 Alistair Forrest
Protein similarity via hydropathy plots? Bernard Murray
world PING CHEN
'Tandem' Primers for PCR Mutagenesis Steve Rodems
Oligo. programs Shiao Y. Wang
Lost posting on His-Tag artifact choie01 at mcclb0.med.nyu.edu
Oligo. programs futers at bpxtal.leeds.ac.uk
cell surface labeling MBWEN at CCVAX.SINICA.EDU.TW
in vitro translation Bernhard Seehaus
How do you make Deionized foramide? Mr. M.J. Lush
Glycoproteins Mauricio Cesar Palmieri
Klenow question Henry
RAIN-X on Sequence Gels? Stephen R. Lasky
Oligo. programs 4700gbera at UMBSKY.CC.UMB.EDU
How do you make Deionized foramide-Mixed bed resin Basavaraju Shankarappa
Wizard/Magic Preps Michael Coyne
Protein similarity via hydropathy plots? Michael Coyne
world Stephen R. Lasky
PET system - alternate plasmids Geethanjali Akula
How do you make Deionized foramide? Michael Coady
"Stabilose K" for Growth of Streptomyces lividans? Dr. Diana S. Hamilton
Availability of Furin HISCOX
Starting material *Anatomo Pathologie
CD/MCD spectral atlas??? kkaye at vax.oxford.ac.uk
Flu-HA tag Sue Jeffrey
'Tandem' Primers for PCR Mutagenesis Bernard Heymann
CD-3 Antibody? littgj at wmvx.lvs.dupont.com
protein expression, PET system Bernard Heymann
DNA Imaging workstation ( ideas) ez002976 at othello.ucdavis.edu
Metaphor Agarose - how is it? 4700gbera at UMBSKY.CC.UMB.EDU
Where did the 2.3 kb band go? Michiaki Masuda
E-mail addr. for Rose Kirwin? Chris Larminie
Rabbit or pig collagen needed Front Office Sun Account
B-Gal and Methyl Salicylate Dave Bates
What is nitrocellulose? m griep
'Tandem' Primers for PCR Mutagenesis Andy
Summary of software for sequence alignment Karyn Davis
chemiluminescent immunodetection Rascals
Quantifying minute amounts of DNA? Rascals
DNA quantitation method wanted Rascals
Drying of PAGE gels - Cellophane Wendy Robinson
Klenow question Stephen R. Lasky
35S in PCR machines.. bahr at plato.oci.utoronto.ca
E-mail addr. for Rose Kirwin? Francis Ouellette
Oligo. programs Francis Ouellette
Allelic Exchange in Bacteria/RainX for Gels Christine Ward
B-Gal and Methyl Salicylate Dr S T May
Microbiology discussion leader needed Dan Diaz
cell surface labeling T. S. Pillay
35S in PCR machines.. Steve Rodems
Protease Inhibitors Craig Marshall
nucleotides assay Knut Eichler
Interesting WWW links for scientists!!!! Martin Leach
How to get Mosaic for WWW browsing Martin Leach
PCR from genomic DNA GEORGE XIAOXI OUYANG, ELECTRICAL ENGINEERING, PHONE 579-0970
'Tandem' Primers for PCR Mutagenesis GEORGE XIAOXI OUYANG, ELECTRICAL ENGINEERING, PHONE 579-0970
??curr.prot. in mol.biol.??-ivo w. Gregory R. Harriman
Wizard/Magic Preps Gregory R. Harriman
stratagene pCR-script--- good or bad? jlanders at hmivax.humgen.upenn.edu
Wizard/Magic Preps Gregory R. Harriman
Interesting WWW links for scientists!!!! Helge Weissig
Methyl salicylate and B-Gal bates
isolation of papilla from mouse hair Hung-Yi Su
Cos ends in cosmids Richard Near
www servers brett at BORCIM.WUSTL.EDU
Help purifying large amounts protein botjlh at lure.latrobe.edu.au
DNA quantitation method wanted nicola fidler
Problems with immunoprecipitation U5267049 at ucsvc.ucs.unimelb.edu.au
'Tandem' Primers for PCR Mutagenesis Oliver Sorgenfrei
How do you make Deionized foramide? M K Bennett
35S in PCR machines.. lmullen at eye1.eye.ufl.edu
35S in PCR machines.. Parke K. Flick
<None> fussenegger at urz.unibas.ch
T3 in vivo expression fussenegger at urz.unibas.ch
Biosafety requirements for Class II organisms meinersmann at BSCR.UGA.EDU
PVA in blocking? SUSI
Help purifying large amounts protein Dan Diaz
Klenow question Steven Coon
Questions: G to A and C to T substitutions, is this a rule ? Zhongguo Xiong
REPEAT:DNA IMAGING WKSTATION ( NEED HELP ) ez002976 at othello.ucdavis.edu
How to get Mosaic for WWW browsing Michael Holloway
TRITC (FITC) labeling Robert Reeves
??CAT assays?? Robert A. Anders
(none) flo at UNIXG.UBC.CA
Enzyme Kinetics Question flo at UNIXG.UBC.CA
Sequencing gel wzhao at mbcr.bcm.tmc.edu
stratagene's pcr script cloning kit russos at HAL.HAHNEMANN.EDU
insect vectors James D DeVault
Reverse transcriptase russos at HAL.HAHNEMANN.EDU
What is nitrocellulose? James B. Hutchins
Metaphor Agarose - how is it? Hank Seifert
Klenow question neale at mbcf.stjude.org
max growth temp for E.coli ? Duncan Cochran -BIOCHEM
non-leaky MMTV plasmids Seth Corey
HOW TO EXTRACT RNA FROM DERMAL PAPILLA OF THE MOUSE Hung-Yi Su
Klenow question Shiao Y. Wang
Human CD4 COS cells? Condie Carmack
subscribe ez002976 at othello.ucdavis.edu
gaskets? dkatz at earth.doane.edu
dextran sulfate-- a cheaper source?? h9290184 at hkucc.hku.hk
What is nitrocellulose? Martin Leach
Green Card Lottery- Final One? Laurence Canter
Help-Mesh jreich at illite.u-strasbg.fr
Klenow question Brian T. Greuel
need reference for pigskin gelatin in tis. culture Bulletin board login
Metaphor Agarose - how is it? Damak, Sami
Metaphor Agarose - how is it? Frances Hannan Zoology
RNase in gel loading buffer recipe? Christopher Coward Path
Max Temp for E. coli Delia Gutman
Differential Display PCR - what is it? Duncan Clark
lambda dna extraction shantaram bharadwaj
Questions: G to A and C to T substitutions, is this a rule ? Olav Hungnes
Help-Mesh Bernard Heymann
RNase in gel loading buffer recipe? Gus Trautweiler
Differential Display PCR - what is it? Basavaraju Shankarappa
Klenow question David L. Haviland, Ph.D.
dextran sulfate-- a cheaper source?? Andrew Cockburn
mineral oil on thermocycler block Marcelo Carnier Dornelas
gelatin in PCR buffer Marcelo Carnier Dornelas
Protein Precipitation Sam Volchenboum
Cloning low abundance PCR products? neale at mbcf.stjude.org
genetic questions ez005679 at hamlet.ucdavis.edu
protein dephosphorylation Geraldine A. New
What services are availible for a fee? Hank Seifert
Direct sequencing of lambda DNA? ajwatson
Looking for T7 polymerase gene Virginia Dress
question regarding RACE kits ajwatson
dextran sulfate-cheap substitute David Ish-Horowicz
Do you have to sequence both strands? Lisa Vawter
Class I MHC NEWLIN_C
Class I MHC NEWLIN_C
Enzyme Kinetics Question Warren Gallin
Difficulty Sequencing Using Internal Primers Christine Ward
Thermocyclers Janet Williams
How do you make Deionized foramide? the End
stratagene pCR-script--- good or bad? nishir at ohsu.edu
What is nitrocellulose? Pierre Jelenc
Klenow question Martin Kennedy
Interrupted reading frame, help! chai_z at wehi.edu.au
Scanning of Agarose Gels Matt McLeod
Decontamination of phage-waste? Peter Kristensen
sequencing gels sticking to plates mmm_jharrison
chemilumpinescent immunodetection, more info please LOGAN
Help with radioactive PCR Dr. M. Rhodes
molecular biology programs Mr V. Schoenfeld DOM
Fluorogenic cathepsin D substrates Philip Fortgens
address for Amgen In. WMGDDM
Software to piece together restriction fragment Tan Shyh Han
NaOH in PCR buffer Fabrice Glibert
PCR probes futers at bpxtal.leeds.ac.uk
Source of Nylon mesh Dr L Malek - Biology
Hybridizations using degenerate oligo's Wayne Beyer
Sequencing gel Dan Diaz
NaOH in PCR buffer Matthias Zeiner
dextran sulfate-- a cheaper source?? Sean David Moore
Methyl Salicylate and B-gal Maureen Ann Torok
DNA&RNA Extraction kits Vijay A. Varma
Silent Mutagenesis: Program wanted Michael J. Tino
Rain-X wzhao at mbcr.bcm.tmc.edu
Ethidium disposal (a VFAQ) Joerg Langowski
Rain-X ZHAOMIN 'THE VICTIM OF SCIENCE'
PCR John Brunstein
Access Wanted--Human Genome Sci Joe Kramer
Glutathione homology Mike Preston
Software to piece together restriction fragment Manuel G. CLAROS
Random Labelling:Sequenase Seth Findley
Sequencing gel Stephen R. Lasky
Rain-X Stephen R. Lasky
PCR Mgr.Jiri Drabek
Trinucleotide repeat Expansion (RED) Edward Stenroos
Protein Precipitation Christopher Cilley
Actin probes in Northerns Adrian Molenaar
re actin probes in northerns Adrian Molenaar
Metaphor Agarose - how is it? Paul N Hengen
Interesting WWW links for scientists!!!! Artur Burzynski
** IMPORTANT ** FTP site for FAQ list changed Paul N Hengen
Oligos labeling Jose_Costoya
FAQ list for bionet.molbio.methds-reagnts Paul N Hengen
Protein precipitation Christopher Cilley
Expression vector for E.coli genes in Bacillus stearothermophilus? Duncan Cochran
Heparinised blood and PCR Scrimshaw, Brian J
Thermocyclers Shiao Y. Wang
syntax for unsubscribe SORIN_D at PAVO.CONCORDIA.CA
Interrupted reading frame, help! gc
Scanning of Agarose Gels gc
RNA dot-blot methods jun ikeda
Run-off/Run-on Martin Leach
Cloning low abundance PCR products? Martin Leach
Source of Nylon mesh Martin Leach
Responses for solubilization of membrane proteins ! G. Jawahar Swaminathan
Oligos labeling daj (David Johnston)
RAIN-MAN Dr. M.D. Jones
b-galactosidase assay after transfection 石塚俊治
Qiaex and ligations LOGAN
Oligos labeling Parke K. Flick
chemical reagent search mazella
Cloning low abundance PCR products David Schuster
GHOST-BAND UN-MASKED BSS032 at BANGOR.AC.UK
Wizard (again ! :) Christopher Coward Path
b-galactosidase assay after transfection Martin Leach
PCR probes Martin Leach
MOPS buffer usage - ? reuse for RNA gels Karen L. Yoas
Need highly expressed clone in LambdaZap Philip L. Carl
Decontamination of phage-waste? Stuart Kuhstoss
Silent Mutagenesis: Program wanted Stuart Kuhstoss
PCR Stephen Scharf
PCR probes Shiao Y. Wang
genomic cloning vectors Shiao Y. Wang
What was hydroxy-lysine? Mr. M.J. Lush
GHOST-BAND UN-MASKED Helge Weissig
Silent Mutagenesis: Program wanted Gary Rudnick
MOPS buffer usage - ? reuse for RNA gels sticknbd at miranda.cc.vanderbilt.edu
PCR from genomic DNA Rajesh R. Naik
mineral oil on thermocycler block Geraldine A. New
Help with radioactive PCR Geraldine A. New
hybridization ovens Richard B. Taylor
PCR Terry-Lynn Young, Medicine, MUN
Trinucleotide Repeat Expansion (RED) Edward Stenroos
PCR from genomic DNA Jim Owens
cDNA libraries Steven Coon
Best source for IL2? Song-Muh Jong
Pharmacia ALF automated sequencer, info wanted. Brian Foley
mineral oil on thermocycler block Steven Coon
What was hydroxy-lysine? Brian T. Greuel
How to get Mosaic for WWW browsing Dennis Benson
MOPS buffer usage - ? reuse for RNA gels Steven Coon
Do you have to sequence both strands? Steven Coon
Need replacement for Wizards Russ Wyborski
Qiaex and ligations Helge Weissig
hybridization ovens Shiao Y. Wang
NIH biosafety guidelines needed Michael Szostak
PCR from genomic DNA krishnan at BORCIM.WUSTL.EDU
Strange agarose gel ethidium bromide staining fading huangbx at DEAKIN.EDU.AU
Strange agarose gel ethidium bromide staining fading huangbx at DEAKIN.EDU.AU
PCR probes Brian Foley
Strange agarose gel ethidium bromide staining fading huangbx at DEAKIN.EDU.AU
Do you have to sequence both strands? (anecdotes) Jim Woodgett
Enzyme Kinetics Question Dan Diaz
PCR from genomic DNA Klaus.Matthaei at ANU.EDU.AU
checking for DNA on nylon membranes? Ron Caspi
EcoRV and MetRV max temperature for activity Duncan Cochran -BIOCHEM
How do you make Deionized foramide? rphjr at nwu.edu
algal DNA purification dunahayt at TCPLINK.NREL.GOV
dextran sulfate-cheap substitute David M. Berman
Probing for small peptides on NCF's? botbml at lure.latrobe.edu.au
Need replacement for Wizards malaine at sara.cc.utu.fi
How to get Mosaic for WWW browsing Duncan Clark
GHOST-BAND UN-MASKED Duncan Clark
PCR probes Mr V. Schoenfeld DOM
Bovine Brain cDNA Library wanted K.M.Chan
What was hydroxy-lysine? David I Resnick
test, pl. ignore ranjit at pasifae.cc.uch.gr
Antisera agaist PSII proteins ranjit at pasifae.cc.uch.gr
hybridization ovens Tony Sanchez
in vitro transcription maiorano at molbiol.ox.ac.uk
BIOCORE Stas Philippe
PCR from genomic DNA Stephen R. Lasky
UV radiation of PCR mixes Morten Lukacs
Sequencing Gel adheres to both plates Ben User Guest
Sequencing Gel adheres to both plates Ben User Guest
Polariod slide maker Graham Atherton
Do you have to sequence both strands? (anecdotes) txpljfg at UABCVSR.CVSR.UAB.EDU
need promoters James D DeVault
How does silver staining work? Bernard Khoo
IBM PC Image analysis program: seek suggestions wzhou at emoryu1.cc.emory.edu
MMTV plasmids Seth Corey
PCR probes Molapo Qhobela
Interesting WWW links for scientists!!!! P A Keller
testkab Kirk A. Bartholomew
Inhibitor of ProteinaseK? BSS032 at BANGOR.AC.UK
Inhibitor of ProteinaseK? Greg Gloor
What is cycling probe reaction ? Basavaraju Shankarappa
PCR Andreas Vogel
Silent Mutagenesis: Program wanted Bonham, Peta
DNA extraction from eggs APARKER at MAINE.MAINE.EDU
Sequencing gel Yolanda Yang
PCR specificity Hayward-Lester
library screening with degenerated oligomers Shu-Chih Chen
Silent Mutagenesis: Program wanted Basavaraju Shankarappa
Silent Mutagenesis: Program wanted Chris Upton
Coupling proteins in presence of SDS? David Nunn
PCR specificity txpljfg at UABCVSR.CVSR.UAB.EDU
UV radiation of PCR mixes NEWLIN_C
Want Heat shock 90kd proteins cDNA WANGNN at UCBEH.SAN.UC.EDU
Sequencing gels Michael Joiner
Coupling proteins in presence of SDS? Rob Steele
Coupling proteins in presence of SDS? David Nunn
E-mail for John Bertin and S. Tajbaksh Brigitte Weston
RE^2:b-galactosidase assay after transfe 石塚　俊治
Where did the 2.3 kb band go? Michiaki Masuda
Glowing Blue DNA Gels Frank Anthony Skraly
PCR probes (32 P) Q&A J. David Spafford
DNA Pro Scan - software ?? ez002976 at othello.ucdavis.edu
Silent Mutagenesis: Program wanted ez002976 at othello.ucdavis.edu
Difficulty Sequencing Using Internal Primers h9290184 at hkucc.hku.hk
PCR probes Shiao Y. Wang
Sequencing gels Shiao Y. Wang
PCR specificity P Watson
PCR probes (32 P) Q&A J. David Spafford
Sequencing gels Robert A. Anders
NEED Amresco ProPage Info! David M. Berman
Nucleic Acid Binding on Syringe Filters dkim at unm.edu
Blocked N-terminus Mark Jason Logan
Polariod slide maker Mark Jason Logan
Supplier for entactin (nidogen)? Brian T. Greuel
Re-POST of THE ORIGINAL MERLIN MINI/MAXI-PREP. Ravi Iyer
Blue-white selection Tom Kristensen
b-galactosidase assay after transfection Robert A. Anders
Re-POST of THE ORIGINAL MERLIN MINI/MAXI-PREP. Ravi Iyer
Nucleic Acid Binding on Syringe Filters Dan Diaz
RE^2:b-galactosidase assay after transfe Dave Bates
ANP C receptor antibodies Dave Bates
Strange agarose gel ethidium bromide staining fading the End
PCR Martin Leach
Peptide Synthesis/ Threonine Analog George W Chacko
Glowing Blue DNA Gels Alistair Forrest
PCR probes (32 P) Q&A Marianna Max
Re-POST of THE ORIGINAL MERLIN MINI/MAXI-PREP Ravi Iyer
DNA extraction from eggs Steven Coon
Software help liyer at bioch.tamu.edu
Polariod slide maker Ola Myklebost
b-galactosidase assay after transfection Michael Kertesz
lambda PCR Przemko
Protein Precipitation John McDougall
Slide makers ANDY PHILLIPS
stable tranfection mazella
Sequencing gels the End
Bacillus transformation shantaram bharadwaj
Protein Precipitation: how about microconcentrators? Basavaraju Shankarappa
Blocked N-terminus Shaun D. Black
Nitrate Reductase Assay Glenda A. Baker
IEF on agarose gels L.Frigerio
Seeking wild-type E. coli strain Roberto Albinoni
Nested Deletion paradox Casey M. Finnerty
advice needed for check trace mRNA GEORGE XIAOXI OUYANG, ELECTRICAL ENGINEERING, PHONE 579-0970
NIH biosafety guidelines needed Marion Freistadt
checking for DNA on nylon membranes? Casey M. Finnerty
does glycogen in RNA sample interfere with Northerns? Rob Steele
Slide makers Leonard N. Bloksberg
Protein Kinase Suppliers? Leonard N. Bloksberg
Silver Stain (Audi 5000 effect) Paul N Hengen
Chemiluminescent 2-D Gels dkim at unm.edu
PCR specificity Klaus.Matthaei at ANU.EDU.AU
Butanol extraction Scrimshaw, Brian J
Glygogen/Microcons and disappearing DNA Scrimshaw, Brian J
Wizard (again ! :) Ron Kagan
Butanol extraction Seth Corey
lambda PCR Shiao Y. Wang
PCR triplet Shiao Y. Wang
Removal of factor X from reaction T. S. Pillay
Removal of factor X from reaction T. S. Pillay
Removal of factor X from reaction T. S. Pillay
Nested Deletion paradox Klaus.Matthaei at ANU.EDU.AU
ligations in LMP agarose Darren Martin
ligations in LMP agarose mazella
in situ oligo design software ? Paul Klosen
proteins sticking to eppendorfs? Denni Schnapp
Tobacco cDNA library LOGAND
PCR triplet txpljfg at UABCVSR.CVSR.UAB.EDU
e-mail address of Dr. Melvin Simon KANYUKA
Oligo design Paul Stanton
Sequencing gel Stephen R. Lasky
NaOH in PCR buffer Wasun Chantratita
butanol extractions russos at HAL.HAHNEMANN.EDU
Glyoxal gels for RNA electrophoresis Michael Joiner
ligations in LMP agarose the End
Brij58 in blocking? Or others SUSI
Oligos labeling Wasun Chantratita
ligations in LMP agarose otello at sc2a.unige.ch
Slide makers Shiao Y. Wang
DNA Probe detecting X and Y chromosomes Wasun Chantratita
lambda dna extraction Bob Darnell
ligations in LMP agarose Bernard Heymann
Metaphor agarose summary 4700gbera at UMBSKY.CC.UMB.EDU
Differential Display : Joann Schnetzer BIO
Glycogen Carrier for SS DNA Michael G. Tencza
Antigenicity of His Tag? Steven Joseph Szarka
Nested Deletion paradox Dave Boyd
Phage DNA prep.-Expected Yield? Robert Roxby
Questions: G to A and C to T substitutions, is this a rule ? Chris Noren
Separation of mutant and wild-type ribosomes Michael van Waes
Looking for pT7-7 cloning vector sequence Shaun D. Black
Total RNA isolation reagent Charlie Watts
PLASMIDS STUCK ON NITROCELLULOSE Philip OBrien
Brij58 in blocking? Or others Klaus.Matthaei at ANU.EDU.AU
sequencing GC-rich fragment Dmitry A. Los
Taq and dITP Robert Slany
ligations in LMP agarose futers at bpxtal.leeds.ac.uk
A NEW NEWSGROUP? bionet.molecular.modelling Stas Philippe
Agrobacterium DNA extraction Giacomini Alessio
ABI sequence assembly software GEORGE MURPHY
Dwindling Plasmid yield *HELP* Martin Leach
Phage DNA prep.-Expected Yield? Duncan Clark
Differential Display : Stephen R. Lasky
Glowing Blue DNA Gels Stephen R. Lasky
ECL immunodetection LOGAN
Taq and dITP mfb102 at CD4680FS.RRZE.UNI-ERLANGEN.DE
Taq and dITP Basavaraju Shankarappa
Glowing Blue DNA Gels Dr S T May
TBE buffer precipitation RMARTINQFD at BIOTECHNET.COM
Reference on 2D gel for DNA sought. Gus Trautweiler
Metaphor agarose summary Clifford J Beall
DNA Probe detecting X and Y chromosomes Wasun Chantratita
advice needed for check trace mRNA Martin Leach
unsubscribe Joseph E Gagnon No relation
locating vector sequences Phil Cunningham ext4239
(none) Pio Donizete A. Zapella
D2-dopamine receptor/cell line PREUSS at MPASMB.DESY.DE
TBE buffer precipitation Martin Leach
Differential Display : Helge Weissig
Ambion wzhao
MacTetrad 6.9...New and Improved! Jonathan Greene
tandem repeat plasmid? Ali Karami
P1 DNA Prep Paul Edward Kowalski
IEF on agarose gels JOHN PROUDMAN
Glowing Blue DNA Gels Paul Haney
Looking for pT7-7 cloning vector sequence Bernard Heymann
inclusion bodies Tom MacAllister
tandem repeat plasmid? Tony Sanchez
Sequencing gels pmiguel at bilbo.bio.purdue.edu
Crystal Violet for Plaque Assay Robert Citek
Storage bact. via Lyophil. Richard Near
Phage prep DNA yield question Robert Roxby
Carbohydrates & Coumarin Detection Steven Striepeke
Glycogen Carrier for SS DNA Paul N Hengen
Temp vs Activity data for EcoRV and MetRV duncan cochran
TBE buffer precipitation fzestabr at chip.ucdavis.edu
lambda PCR chai_z at wehi.edu.au
PCR probes Lynda Callicotte
PFGE Wei C.
TBE buffer precipitation Klaus.Matthaei at ANU.EDU.AU
inclusion bodies Bernard Heymann
Ambion Martin Leach
Test u5267049 at ucsvc.ucs.unimelb.edu.au
HI HO SILVER Philip OBrien
39-mer primers Jenny Witherspoon
39-mer primers Jenny Witherspoon
interactive multimedia mol biol teaching robert at chironex.jcu.edu.au
Plate Washer Interest GVRIVER at DELPHI.COM
Silver Stain (Audi 5000 effect/All at once) rhubner at molbiol.ox.ac.uk
Problems with SDS-Page botanica at icil64.cilea.it
PCR rhubner at molbiol.ox.ac.uk
Glowing Blue DNA Gels Eric R. Hugo
Help wanted staining DNA gels Jackie Fetherston
TBE buffer precipitation Jim Owens
Oligo design Stephen R. Lasky
Differential Display : David M. Berman
Dirty PCR! txpljfg at UABCVSR.CVSR.UAB.EDU
ligation in agarose Callum Bell
Problems with SDS-Page Shaun D. Black
interactive multimedia mol biol teaching Naresh C. Pancholi
PFGE Andrew P. Miller
Problems with SDS-Page bss032 at vaxa.bangor.ac.uk
advice needed for check trace mRNA James S. Sutcliffe
a couple things Warren Gallin
a couple things Henry
2,4-DCP Mati Marits
Crystals during X-gal staining Martin Leach
a couple things Henry
ABI sequence assembly software John Watson
D2-dopamine receptor/cell line John Watson
e-mail address of Dr. Melvin Simon John Watson
Dirty PCR! Jian_Fu Wang
Crystals during X-gal staining Allison Haggarty
Differential Display : Helge Weissig
A protein question****** Shahram Mori
capillary PCR Mr V. Schoenfeld DOM
recent 96-well-plate PCR discussion John Alsobrook
Antibodies Pio Donizete A. Zapella
HI HO SILVER dkim at unm.edu
library screen problem JOHN
Help wanted staining DNA gels Paul N Hengen
PCR: hot start Basavaraju Shankarappa
Phage prep DNA yield question Steven Coon
checking for DNA on nylon membranes? Lynda Callicotte
TBE buffer precipitation Mike
Crystals during X-gal staining Basavaraju Shankarappa
Biotinylated primers Shiao Y. Wang
Problems with SDS-PAGE JOHN PROUDMAN
PCR: hot start Helge Weissig
Crystals during X-gal staining Bernard Murray
Has anyone used Epicentre's Ready-lyse lysozyme? Roberto Albinoni
Reference on 2D gel for DNA sought. Rafa Maldonado
Retics and DNA binding proteins Steve Rodems
TBE buffer precipitation the End
Crystals during X-gal staining Martin Leach
Nested Deletion paradox Paul A Bucciaglia
a couple things Henry
Retics and P'ation Steve Rodems
WORKSHOP & MUCL CENTENARY Moulliard
inclusion bodies Peter Gegenheimer
Wanted: lambda EMBL3/EMBL4 sequences knoblauch at mpimg-berlin-dahlem.mpg.de
PCR book/manual Michael Joiner
Problems with SDS-PAGE txpljfg at UABCVSR.CVSR.UAB.EDU
methods to prevent digestion by REs? Basavaraju Shankarappa
Non-radioactive oligos for Southerns Dave Knecht
Wanted: lambda EMBL3/EMBL4 sequences futers at bpxtal.leeds.ac.uk
Dwindling Plasmid yield *HELP* Steven Coon
Sequencing gel Steven Coon
Wanted: lambda EMBL3/EMBL4 sequences futers at bpxtal.leeds.ac.uk
Enzyme Kinetics Question Ray Deems
Crystals during X-gal staining Paul N Hengen
library screen problem Andrew Cockburn
Possible ghost plasmids available for study Paul N Hengen
Sequencing gel Stephen R. Lasky
Qiagen Oligotex mRNA kits? lsalati at wvnvms.wvnet.edu
Reference on 2D gel for DNA sought. John H McDonald
Metaphor agarose summary HYNEK WICHTERLE
unsubscribe krishnan at BORCIM.WUSTL.EDU
A protein question****** Virginia Dress
Phage display of Small Polypeptides flo at UNIXG.UBC.CA
Biological ClipArt flo at UNIXG.UBC.CA
Silent Mutagenesis for Macs flo at UNIXG.UBC.CA
??CAT assays?? Uttam Rajbhandary
Biological ClipArt Martin Leach
Problems with SDS-Page Klaus.Matthaei at ANU.EDU.AU
test u6069416 at ucsvc.ucs.unimelb.edu.au
Problem to attend ASBMB meeting BMKAO at CCVAX.SINICA.EDU.TW
Protein Precipitation u6069416 at ucsvc.ucs.unimelb.edu.au
methods to prevent digestion by REs? Steve Rodems
PCR book/manual David M. Berman
Inverse PCR Joshua Daniels
PCR enhancers Bratty John
Archive Searches Patricia Foster
PCR book/manual none
Wanted: lambda EMBL3/EMBL4 sequences Francis Ouellette
PLASMIDS STUCK ON NITROCELLULOSE Shahram Mori
Protein Precipitation Doug Portman
Archive Searches Richard Near
Crystal Violet for Plaque Assay Marion Freistadt
Stringent Lac promoter control KRUEGER, CHARLES M
ECL immunodetection Stacy Ferguson
Test Ajay Bhatia
Test-please ignore. （送信試験） M. Yamagata （山形）
Re-POST of THE ORIGINAL MERLIN MINI/MAXI-PREP. Bruce Roe
Protein Precipitation Stephen R. Lasky
UK distributor of GCK Brian Smith Bioc
Inverse PCR Martin Leach
radioactive 5'label.mRNA? Martin Leach
radioactive 5'label.mRNA? Schu Bernhard
(none) krishnan at BORCIM.WUSTL.EDU
Nested-deletions paradox Dave Boyd
Inverse PCR Greg Denomme
Stratagene Zap Express Vector Val Snewin
Removal of factor X from reaction Tom Morrison
Testing Joseph C. Bagshaw
luminometer sources Xiu Yu
Info request: Darwin Molecular Technologies Kieron Des
Temp. for DNA/glasswool binding? Ed Beaty
ANCHORED PCR txpljfg at UABCVSR.CVSR.UAB.EDU
Glasswool for DNA purification-how nasty is it? Basavaraju Shankarappa
re;bioimages john hachey
Temp. for DNA/glasswool binding? dkim at unm.edu
Where can I get Difron fzestabr at chip.ucdavis.edu
PCR book/manual Randy P. Rasmussen
Wanted: lambda EMBL3/EMBL4 sequences Martin Kennedy
methods to prevent digestion by REs? Rapu Lak
Dwindling Plasmid yield *HELP* David L. Haviland, Ph.D.
Dwindling Plasmid yield *HELP* Martin Leach
Software to piece together restriction fragment Allison Hanly
UK distributor of GCK Allison Hanly
Access Wanted--Human Genome Sci Nathan Caffo
Source for Tobacco Acid pyrophosphatase Terry Hanzlik
glycerol inPCR LEES at SASK.USASK.CA
absorbance of CTAB LEES at SASK.USASK.CA
Good RACE and PCR cloning kits? Timo Hiltunen
Good RACE and PCR cloning kits? Matthias Zeiner
Thermal Cyclers Dr. M.D. Jones
Conjugation Strains? plxigpg at vax.ccc.nottingham.ac.uk
Biotinylated anti-rat CD2 (OX34?) Martin Rowe
Dwindling Plasmid yield *HELP* David Huen
UK distributor of GCK David Huen
cycle sequencing Rick Wilson
PDesperately seeking pBCKS minus I.B. Vipond
Phage library contamination Matthias Zeiner
Glasswool for DNA purification-how nasty is it? Stephen R. Lasky
Retics and DNA binding proteins Bill Jack
methods to prevent digestion by REs? Bill Jack
luminometer sources littgj at wmvx.lvs.dupont.com
Strange agarose gel ethidium bromide staining fading Bill Jack
Dwindling Plasmid yield *HELP* Martin Leach
oligo synthesizer&thermocycler jurgenson at cobra.uni.edu
Help w/ Seq. Primer for pATH vectors Loren Joseph
RT-PCR Steven Coon
plasmid carrying lambda cI+ ??? Joel Caillet
plasmid carrying lambda cI+ ??? Joel Caillet
Glasswool for DNA purification-how nasty is it? Andrew Cockburn
SV-40 promoter David Huen
some asshole brett at BORCIM.WUSTL.EDU
dialysis membrane for low MW Denni Schnapp
b-galactosidase assay after transfection David Huen
Purify soluble bacterial expressed protein WANGNN at UCBEH.SAN.UC.EDU
ISHIKAWA cell line T P Ramkumar
CBB-Binding Assay ! murugan at tifrvax.tifr.res.in
Assay for SIALIC ACID ? murugan at tifrvax.tifr.res.in
Making fusion proteins with protein kinase sites for 32P labelling Simon Kidd
Help wanted staining DNA gels wmelchior at fdant.nctr.fda.gov
Jack bean cDNA or genomic library Roland Contreras
5'-RACE: Kits vs "homemade" reagents Anitra S. Auster
RE - Bsi I or Rle AI HYNEK WICHTERLE
Mouse L cell cDNA library Stephan Spencer
Hydrophobic Interaction Chromatography BARRY J MARGULIES
Tobacco enzyme Wachara Kasinreak
RT-PCR Martin Leach
5'-RACE: Kits vs "homemade" reagents Martin Leach
Tobacco enzyme Martin Leach
acid-denatured enolase Seth Corey
Sequences needed Jane Harrison
Making fusion proteins with protein kinase sites for 32P labelling T. S. Pillay
Protein Kinase Suppliers? T. S. Pillay
dialysis-low MW T. S. Pillay
Culture contaminations WAS: Dwindling Plasmid yield *HELP* Allison Hanly
lambda dna extraction G Turner
3'ECL(Amersham) background Laksana Kantama - RAPA
Wanted: lambda EMBL3/EMBL4 sequences knoblauch at mpimg-berlin-dahlem.mpg.de
Activation and attachment to glass beads B.G.Macdonald
SV-40 promoter Matthias Zeiner
RE - Bsi I or Rle AI Duncan Clark
Phage library contamination Duncan Clark
Culture contaminations WAS: Dwindling Plasmid yield *HELP* Martin Leach
Glowing Blue DNA Gels Dennis J. Templeton
Using glycerol to enhance PCR amplification. Wasun Chantratita
Phage library contamination Matthias Zeiner
Problems with SDS-Page Sean David Moore
How can we directly sequence PCR-amplified DNA from agarose. Wasun Chantratita
TBE buffer precipitation Jim Owens
Colloidal Gold and Nanogold xuxian
Subscription termination krishnan at BORCIM.WUSTL.EDU
(none) sjjdlli
PCR: hot start Hank Seifert
Request for GeneReleaser matrix supplier WILLIS at BCRSSU.AGR.CA
Gene-Amp RNA PCR Kit Steven Coon
Software to piece together restriction fragment Manuel G. CLAROS
DNA extraction systems WILLIA48 at CCVAX.MMC.EDU
HELP: Stripping western blots Kevin Morano
Where can I get Difron Manuel Simon
Good RACE and PCR cloning kits? Manuel Simon
Problems with SDS-Page Manuel Simon
Anybody interested in this idea? Kieron Des
Sequences needed Michael Poidinger
(none) Dan Guerra
Binding Sialic Acid Paul T. Englund
Genereleaser info John Brunstein
absorbance of CTAB JONATHAN GARY
In vitro UV Mutagenesis 5978jsnyd at UMBSKY.CC.UMB.EDU
subscription g9818tdooc at UMBSKY.CC.UMB.EDU
RUN-OFF REFS AND INFO **WANTED** Martin Leach
Anybody interested in this idea? Martin Leach
pET-26b pelB Leader? Patrick G. Durham
RNA binding to hela nuclear proteins Dorian Winter
Retroviral vector search Graham Atherton
Useful Doc for Auto-Sequencer Users Gene Codes
A protein question******close dallas at mcvax4.d48.lilly.com
Software to piece together restriction fragment Jason Rancourt
DNA extraction from formalin-fixed material Riccardo Fodde
Computer assisted amino acid analysis Uwe Hobohm
Computer assisted amino acid analysis Uwe Hobohm
long PCR's reference jr at dna.bio.warwick.ac.uk
HELP: Stripping western blots Manuel Simon
Subscription termination Manuel Simon
a good alternative to wax for hot starts... Basavaraju Shankarappa
long PCR's reference (fwd) Robert Preston
RT-PCR Jonathan Hecht
Good RACE and PCR cloning kits? John Watson
RT-PCR John Watson
5'-RACE: Kits vs "homemade" reagents John Watson
Hot Start (Wax Beads) Hank Seifert
long PCR's reference John Watson
PCR: hot start Greg Denomme
Dwindling Plasmid yield *HELP* David L. Haviland, Ph.D.
Commercial sources for antibodies to TrpE or polA Roland J Saldanha
S. typhim. LPS needed Michael Szostak
MAP peptides for Abs Tom Doak
bad taste brett at BORCIM.WUSTL.EDU
Tobacco enzyme or a host of others
a test, ignore please. mbchao at ccvax.sinica.edu.tw
E. coli cell extracts? mbchao at ccvax.sinica.edu.tw
test Leon Parker
Vaccinia virus/Expression Ashu Sharma
Announce: A new WWW interface for Molecular Sequence Searching Dan Jacobson
Sequencing gels L. Sumoy
A protein question****** Chanpen Wiwat - PYMI
RT-PCR in paraffin embedded tissue sections Sujatha Byravan
Help:differential display technique smpoke at eagle.wesleyan.edu
How can we directly sequence PCR-amplified DNA from agarose. Wasun Chantratita
Fusion proteins David L. Haviland, Ph.D.
Phage library contamination Shiao Y. Wang
oligo synthesizer&thermocycler Shiao Y. Wang
Glowing Blue DNA Gels Jason Rancourt
How can we directly sequence PCR-amplified DNA from agarose. Shiao Y. Wang
RT-PCR Jinling Xie
Problems with immunoprecipitation U5267049 at ucsvc.ucs.unimelb.edu.au
Current Protocol in CDROM Prasit Palitpolkanpim - GRSO
Cheap device for gel temp Shiao Y. Wang
MAP peptides for Abs Bernard Murray
Electropor./conjug. in Pediococcus Giacomini Alessio
Problems with SDS-Page Sujatha Byravan
PCR from hair follicles/buccal(sp?) cells BOWDEN at MGRP.BGSM.WFU.EDU
Longest dideoxy sequencing read? Bill Nowatzke
Progenitor, Inc. Dr. Jay L. Rothstein
MRC probe repository? Mike Morris
RT-PCR Jonathan Hecht
Wanted: anti-mouse heparanase antibodies Rory Morty
halo/thermos genomic library needed Wolfgang Wegloehner
PCR: hot start Hank Seifert
Longest dideoxy sequencing read? Martin Leach
How can we directly sequence PCR-amplified DNA from agarose. Hank Seifert
Glowing Blue DNA Gels (fwd) Robert Preston
Electropor./conjug. in Pediococcus Hank Seifert
RT-PCR russos at HAL.HAHNEMANN.EDU
Differential Display Robert A. Anders
Current Protocol in CDROM Steven Coon
Longest dideoxy sequencing read? Steven Coon
Probe for p53 Marc J. Mass
Cheap device for gel temp ww40
pET-26b pelB Leader? Michael Benedik
DDRT-PCR Mike Rott
Chromosomal DNA extraction from amoebas Joe Crouse
ampliwax alternatives brett at BORCIM.WUSTL.EDU
RT-PCR in paraffin embedded tissue sections David M. Berman
Northern Blot Background Scott Bearden
Help for fax no. Prasit Palitpolkanpim - GRSO
Longest dideoxy sequencing read? Shiao Y. Wang
Stopcodons in phage fusevectors ? Svend Kjaer
TA microsatellite Hugo Volkaert
Sequencing PCR Products Michael G. Tencza
Wanted: E. coli DH5alphaMCR sstrain_ssb at opal.tufts.edu

Last message date: Sat Apr 30 18:36:48 EST 1994
Archived on: Sun Mar 6 20:05:56 EST 2005

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