PCR and Strand Displacement

Parke K. Flick ch994 at cleveland.Freenet.Edu
Mon Aug 1 12:40:13 EST 1994


As shown by David Gelfand, Taq possesses a polymerization-
dependent 5'-3' exonuclease activity which can degrade DNA 
strands ahead of the enzyme during strand copying.  Taq is,
therefore, a repair type polymerase like E. coli Pol I and 
can strand displace.
P. Flick
-- 
Parke K. Flick, Ph.D.  ch994 at cleveland.freenet.edu
USB/Amersham Life Sciences, Inc.
Cleveland, OH  44128  Ph: 216-464-9277; FAX: 216-360-0975



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