Non EtBr DNA stain(s)
Sebastian W. Bunka
seb at i102pc1.vu-wien.ac.at
Tue Aug 2 04:53:46 EST 1994
Robert Horton (horton at molbio.cbs.umn.edu) wrote:
: Richard Near (rocket1 at bu.edu) wrote:
: : I am interested in DNA staining in gels *not* using ethidium\
: : bromide. I would be most interested in stains that are
: : colorometric. My motivation is that I would like to scan
There was a thread several weeks ago...
I'll give you a copy:
In article <01H9UED2XZKI009ZC9 at Post-Office.UH.EDU>, pedemonte at JETSON.UH.EDU (carlos h. pedemonte) says:
>Has anybody used the SYBR GREEN I nucleic acid gel stain (S-7567) from
>Molecular Probe? The company claims that this dye is 10 times more
>sensitive than Ethidium bromide. The company does not send samples and the
>price is high. Therefore, I would like to have some information before I
>invest the money. I would appreciate any comment.
>Carlos H. Pedemonte Telephone: 713-743-1211 (office)
>Dept. of Pharmacology 713-743-1228 (lab)
>University of Houston Fax: 713-743-1229
>Houston, TX 77204-5515 e-mail: pedemonte at jetson.uh.edu
>l have tested out the SYBR Green I from Molecular probes. I have found that the dye can
be form 100 to 1000 times stronger (in a 254 nm transilluminator) than Ethidium Bromide on DNA probes or PCR products
from 40 to 200 basepairs. On larger DNA the SYBR Green I stain from 20 to 100 time stronger than
Ethidium Bromide. We have found that there is close to no background when using this dye.
THIS DYE IS A REVOLUTION!!! FOR THE PCR TECHNOLOGY.
I have also more informasjon. My telephone is in Norway 47 22 93 41 94, Fax
47 22 73 01 64.
I don't have any more information, sorry - Sebastian
[ Sebastian.Bunka at vu-wien.ac.at ]
Location: earth, europe, austria, vienna Inst. of Bacteriology Vet.Univ.
More information about the Methods