Cloning into Afl III site in pUC based plasmids

Ward Lab Ward_Lab at tikal.biosci.arizona.edu
Thu Aug 4 22:33:21 EST 1994


In Article <85291833wnr at genesys.demon.co.uk>, Duncan at genesys.demon.co.uk
(Duncan Clark) wrote:
>Hi Folks,
>
>Does anyone know if one can use the Afl III site in pUC based plasmids. 
>From maps it is supposedly after the ori but I cannot find anything 
>definite in Genbank etc.
>
>Many thanks
>
>Duncan 
>
>-----------------------------------------------------------------------------
>Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
>GeneSys Ltd.                        | Compuserve:  100015.1406 at compuserve.com
>-----------------------------------------------------------------------------
>
I've used the one in pBluescript to clone into.  I can mail you the sequence
if you email me.  Gotta go home now.
Paul
--------------------------------------------------------------------------------
                                                                               
    Paul J. Muhlrad                                                            
    Department of Molecular and Cellular Biology                               
    University of Arizona                                                      
    415 Life Sciences South                                                   
    Tucson, AZ  85721                                                          
                                                                              
    tel (602)621-9135
    fax (602)621-3709
    email  Ward_Lab at tikal.biosci.arizona.edu

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