CIPPers for breakfast

Carlisle Landel landel at helios
Thu Aug 4 16:15:40 EST 1994


In article <31pl7i$pmp at agate.berkeley.edu>,
Pendragon <lab_winoto at maillink.berkeley.edu.> wrote:
>In article <30orik$qj1 at mserv1.dl.ac.uk>
>"Matthias Zeiner" <mzei at sun0.urz.uni-heidelberg.de> writes:
>
(snip)
>> run the reaction in an appropriate buffer. To my knowledge CIP requires 
>> Zn++ Ions, which are not contained in the Boehringer H buffer.
>
>The NEB buffers also don't contain Zn++ ions but they work just fine
>for NEB cip.  I've made panels of plasmids cut with various polylinker
(snip)

CIP doesn't need Zn.  It works super-well in KGB buffer.  If you are 
digesting using KGB, do the digestion, then add CIP and incubate for
a few (<5 or else!) minutes.

Carlisle Lande;




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