Hi is there anyone had success on site-directed mutagenesis using Amersham's new kit ( sculptor: M13 system )? We used to make mutants using its old version it worked well. But the new kit just did not work. Anyone has some tips? Thx in advance. Yi Dept of Physiology and Biophysics Case Western Reserve University Cleveland, OH 44106 yxd3 at po.cwru.edu