RNA gels: Autoclaving MOPS buffer?
ttha at troi.cc.rochester.edu
Thu Aug 4 08:15:54 EST 1994
In <Aug4.024856.42670 at acs.ucalgary.ca> ktetro at acs.ucalgary.ca (Kelly Tetro) writes:
[Should MOPS be autoclaved or filter sterilized?]
>Pete, I don't sterilize my MOPS buffer (the one from Maniatis)
>and have gotten success (knock on wood and pray not to offend the
>gods of science). I do make up the buffer in a sterile bottle and
>use sterile reagents as much as possible. Any suggestions on why
>it would need to be sterile? Won't RNAases pass through a filter
>and I thought RNAases weren't inactivated by heat?
Since MOPS contains carbon and nitrogen, it (and all the Good buffers)
are potentially tasty snacks for bacteria or fungi...
University of Rochester
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