Sequencing from boiling mini-preps?

Jean Valjean sm010b at uhura.cc.rochester.edu
Fri Aug 5 11:07:32 EST 1994


Hello,
	I have a large set of deletion mutants that I need to sequence, and
I was wondering if it is possible to get sequencing quality DNA off boiling
mini preps.  Does anyone have any experience with this?  If so, what
protocol do you use and how good are the results.  Thanks Alot.


Sean J. McMahon
University of Rochester
Department of Biochemistry
sm010b at uhura.cc.rochester.edu



More information about the Methods mailing list