'Leaky' IPTG inducible promoters...

Duncan Clark Duncan at genesys.demon.co.uk
Fri Aug 5 08:32:13 EST 1994


Have a look at the latest Gene , I think 145 no. 1. There is a paper 
presenting a modified vector to get around this. Basically put laciq on 
the vector and a terminator just upstream of the trc promoter. I 
think it isn't just that the promoter is leaky but there is read 
through from another plasmid encoded promoter that causes the 
problem. You will also find that yeast extract contains an inducer. 
Glucose in the media will help repression. 
  
Duncan
-----------------------------------------------------------------------------
Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
GeneSys Ltd.                        | Compuserve:  100015.1406 at compuserve.com
-----------------------------------------------------------------------------




More information about the Methods mailing list