Osmotic Lysis of Erythroid cells - help wanted!

Mr. A.F. Hill ahill at hgmp.mrc.ac.uk
Sat Aug 6 10:58:36 EST 1994


hi

i am trying to lyse some erythroid cells and purify a protein from the
membrane - basically i want to wash the membrane in pbs and get rid of any
soluble proteins then solubilise my protein of interest from the membrane with
a non-ionic detergent.

i have tried lysing in water but get a clump of cells which i cant do much 
with! has anyone out there had any experience with this sort of prep?

any help would be much appreciated

Andy 




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