PNGaseF

SPAGNOL at PLAUTO.CSATA.IT SPAGNOL at PLAUTO.CSATA.IT
Sun Aug 7 15:37:44 EST 1994


Dear netters,
I'm looking for a good protocol for deglycosilating a glycoprotein with 
PNGase F. The one in Current Protocols for M.B. did not work in my hands and
is also a little bit imprecise.For istance it says: add 100 mg of the protein, without specifiyng in what should it be diluted. Pleas post to me by E-Mail or tothe Newsgroup, and I will compile a summary. Thanks.
Giorgio Spagl
Sorry, Giorgio Spagnol
SPAGNOL at PLAUTO.CSATA.IT



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