antibody production

Peter J. Crilly Peter at compo.demon.co.uk
Sun Aug 7 11:47:53 EST 1994


In article <31ie0s$da9 at mserv1.dl.ac.uk>
           un691cs at genius.embnet.dkfz-heidelberg.de
           "Clemens Suter-Crazzolara" writes:

> 
> dear colleagues,
> 
> i am on the verge of putting my protein into a rabbit with the help of
> the titer-max adjuvans.
> however, at high concentrations, the protein precipitates out ! what
> is the best way to keep it in solution ? add SDS ? i would like to 
> raise antibodies against the native and active protein however.
> 
> clemens, inst anatIII, heidelberg, germany
> 
> 
Perhaps it won't matter. I've raised specific antibodies, both poly- and mono-
clonal, to precipitated antigens, proteins ans protein-hapten conjugates. If
you have enough of you antigen (O.K. immunogen) and a rabbit available why not
try immunising the rabbit anyway?

Good luck.
-- 
Peter J. Crilly



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