disappearing bands

[Frederick Garbrecht]

When I visualize PCR products on agarose gels (0.5x TAE,  ethidium 
bromide),  about 1 out of 10-15 of the gels has no bands, not even 
the DNA marker ladder bands.  If I re-do the gel from scratch, 
usually it looks OK (I get the expected bands).  Probably something 
really stupid is going on, but I am baffled.  Anyone else have this 
kind of dumb problem?

Frederick C. Garbrecht
fred at bmt.mcw.edu
fgarbrec at post.its.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax   414 257 7994