Altered Sites -Pros? Cons?

jcuozzo at jcuozzo at
Mon Aug 8 22:19:19 EST 1994

I am about to start a project that will involve making numerous
point mutations in a certain protein, and I have been leaning
towards using Promega's revised Altered Sites protocol.  Is there
anyone out there who has used this kit, successfully or otherwise?
I would really appreciate your insight. Also, if anyone has any
recommendations or warnings about using other double-stranded
site-directed mutagenesis kits, they would also be much appreciated.

J. Cuozzo
jcuozzo at

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