Cycle sequencing of PCR products

Mr V. Schoenfeld DOM vs10005 at crc.ac.uk
Mon Aug 8 14:13:40 EST 1994


A lot of people seem to be using kits to clean their
PCR products prior to manual/ABI sequencing. That's all
very nice but it can be done cheaply, as I mentioned in an earlier
post, with PEG: 10 minRT, 30 min spin, EtOH wash, TE. It works
very well with cycle sequencing (400-450 on ABI at its best).
Try it!

Vince
vs10005 at med.cam.ac.uk
Cambridge, UK



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