E.coli strains with no lacZ, anyone have such a beast?

szcooley at chip.ucdavis.edu szcooley at chip.ucdavis.edu
Tue Aug 9 10:44:37 EST 1994


tao at mbcf.stjude.org wrote:
: Dear Netters:

: One more question about lacZ.  Does any one have a E.coli strain that have
: most of the lacZ gene deleted that I can have?  Some pointer on such a strain
: would also welcome.

: TIA  for the replies.

: Tao

You should be able to cure a JM101 ,etc, of the F' using acrydine Orange. 
I'll bet it even happen spontaneously at a resonable frequency. Screen 
for the inability to grow in the absence of proline.


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