Osmotic Lysis of Erythroid cells - help wanted!

DjayR djayr at aol.com
Tue Aug 9 18:27:02 EST 1994


In article <320bvc$f7c at mserv1.dl.ac.uk>, ahill at hgmp.mrc.ac.uk (Mr. A.F.
Hill) writes:

I'm not sure this is what you want but we use a 0.83% NH4Cl solution (20
mM Koac, 5 mM EDTA) for lysing red cells when isolating blood or bon
marrow.  This is , of course, and ionic lysis.

Hope it helps
Jay Rothstein
Thomas Jefferson University
rothstei at calvin.jci.tju.edu




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