E.coli strains with no lacZ, anyone have such a beast?

Mark Farinha mfarinha at gpu.srv.ualberta.ca
Tue Aug 9 14:13:50 EST 1994


In article <1994Aug8.124613.8721 at mbcf>, tao at mbcf.stjude.org says:
>
>Dear Netters:
>
>One more question about lacZ.  Does any one have a E.coli strain that have
>most of the lacZ gene deleted that I can have?  Some pointer on such a strain
>would also welcome.
>
>TIA  for the replies.
>
>Tao
>
any of the JM even series will do i.e.( JM102, 104 ...) or JM83, MC1061
these do not have the F' episome

some biotech companies will ship glycerol stocks free with a cold purchase
e.g. (NEB or Promega)

if not i think i have JM106 which you can have.

Mark Farinha
University of Alberta   Biological Sciences



More information about the Methods mailing list