Oligo Cloning

Ben-Sapiens srutt at beagle.Colorado.EDU
Wed Aug 10 17:10:12 EST 1994


Hi, I'm wondering if anyone has tried (and succeeded) subcloning a
ss oligo into a ds plasmid similar to the cutting and ligation step
diagrammed below:

    CATG................................CATG
...C                   |                    C...
...GGTAC               V                GTACG...
   NcoI                                  SphI

There have been a few papers describing this method, but I was curious
if anyone had perfected the technique to the degree that a large
library of randomized sequence could be made. I would appreciate any
and all suggestions. Thanks,

steve srutt at boulder.colorado.edu




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