373A-stretch survey

[hollingswor at immunex.com]

A 373A survey
We're planning on purchasing the Applied Biosystems/Perkin Elmer
373A automated sequencer with the stretch upgrade.  I am trying to
work out schedules for running two gels a day.  I know what AB
says for run times and length of read, but what is reality?
Well-to-Read	Run time	Bases
48cm
34cm
24cm
Chemistry?
ss or ds
Taq or Sequenase
dye-terminators or primers
We do two types of projects that lend themselves well to the two gel
a day concept...type one wants the longest unambiguous sequences and
type two, point mutations.