373A-stretch survey

hollingswor at immunex.com hollingswor at immunex.com
Wed Aug 10 17:50:11 EST 1994


A 373A survey
We're planning on purchasing the Applied Biosystems/Perkin Elmer
373A automated sequencer with the stretch upgrade.  I am trying to
work out schedules for running two gels a day.  I know what AB
says for run times and length of read, but what is reality?
Well-to-Read	Run time	Bases
48cm
34cm
24cm
Chemistry?
ss or ds
Taq or Sequenase
dye-terminators or primers
We do two types of projects that lend themselves well to the two gel
a day concept...type one wants the longest unambiguous sequences and
type two, point mutations. 



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