Altered Sites -Pros? Cons?
Simon E Plyte
splyte at ocicl.oci.utoronto.ca
Wed Aug 10 18:05:29 EST 1994
In article <1994Aug8.221919.1 at opal.tufts.edu>, jcuozzo at opal.tufts.edu wrote:
> I am about to start a project that will involve making numerous
> point mutations in a certain protein, and I have been leaning
> towards using Promega's revised Altered Sites protocol. Is there
> anyone out there who has used this kit, successfully or otherwise?
> I would really appreciate your insight. Also, if anyone has any
> recommendations or warnings about using other double-stranded
> site-directed mutagenesis kits, they would also be much appreciated.
> J. Cuozzo
> jcuozzo at opal.tufts.edu
I have used the old and new promega mutagenesis kits numerous times with
great success. However, I failed to get any mutants using the double stranded
technique and would recommed sticking to the ssDNA method.
More information about the Methods