Gelatin as blocking agent in Westerns

awb at aber.ac.uk awb at aber.ac.uk
Thu Aug 11 04:09:52 EST 1994


In article <phef-1008941211590001 at bglab_1.botany.duke.edu> phef at acpub.duke.edu (Peter Heifetz) writes:
>From: phef at acpub.duke.edu (Peter Heifetz)
>Subject: Gelatin as blocking agent in Westerns
>Date: Wed, 10 Aug 1994 12:11:59 -0400

>Can anyone post their experiences pro/con of using Sigma fish gelatin as a
>blocker in Westerns versus dry milk?

>Thanks,

>-- 
>Peter Heifetz
>Duke University
>Developmental, Cell, and Molecular Biology Group

We use it all the time, mainly as we found it gave us lower backgrounds when 
compared to BSA.  We use gelatin supplied by Sigma (obtained from cold-water 
fish). (1%-3% in a suitable buffer).  The downers seem to be that your gloves 
(or fingers!) can get sticky and smell of fish!

Andy Berry
Biological Sci
UWA 
UK
awb at uk.ac.aber



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