SDS removal

Timothy Bushnell bushnt at rpi.edu
Fri Aug 12 06:33:28 EST 1994


In Article <CuB7Ds.5pF at liverpool.ac.uk>, agmclen at liv.ac.uk wrote:
>I am sure this must be an FAQ, but does anyone have an opinion on the best way
>to remove unbound and bound SDS from proteins recovered from a preparative SDS
>polyacrylamide gel that is mild enough so that significant renaturation and
>recovery of enzyme activity can ultimately be achieved?
>Thanks 

Have you tired dialysis against a buffer.  It has been my experience that
this will ususally work.
Timothy Bushnell
Trying to keep the faith.

"Just the place for a snark," the Bellman cried with glee.
L. Carrol



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