ds sequencing Quiagen plasmid preps

plxigpg at vax.ccc.nottingham.ac.uk plxigpg at vax.ccc.nottingham.ac.uk
Sat Aug 13 08:42:20 EST 1994


>When we try the same protocol on DNA purified using a Qiagen maxi
>prep, it doesn't work, ie, no bands at all.  DNA that is too pure
(stuff deleted)


We have just recently swapped from Magic maxi preps to Qiagen maxi's
I have sequenced my preps twice since then both times with good results.
We use the Sequenase system and denature the DNA using 1M NaOH (1ul) for 5 mins
at 42C then neutralize with 1ul 1M HCl.
Hope this is of some help.

Ian

Plxigpg at vax.ccc.nottingham.ac.uk



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