Pfu Poly, Cloning

ez001427 at dale.ucdavis.edu ez001427 at dale.ucdavis.edu
Sun Aug 14 01:41:53 EST 1994


I've noted some discussions about cloning of Taq-amplified PCR products.  
Since encountering some fidelity problems with Taq, I have been using the 
Pfu polymerase.  I noticed that my blunt-cloning efficiency went up by a 
large factor after  the switch, presumably due to Pfu's lack of tt 
activity.  I often wonder why most people still use Taq, even though it 
is error -prone and has that irritating tt activity. Does Taq have any 
advantages other than price and availability?

Marc Goldstein
magoldstein at ucdavis.edu



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