Quick Coomasie Destain

Allison Haggarty ar229 at FreeNet.Carleton.CA
Mon Aug 15 09:19:31 EST 1994



In a previous article, flh at mole.bio.cam.ac.uk (Frances Hannan Zoology)) says:

>gpkv01 at udcf.gla.ac.uk (John McGeehan) writes:
>
>>Hi folks,
>>	you may have heard of this already but if not...
>>To speed up destaining Coomasie Blue stained gels try the following;
>>1. Take a tea bag, the ones with the string are generally best,
>>2. Break the seal at the top and empty out the tea,
>>3. Fill teabag with an ion-exchange resin eg.Amberlite (mixed bed 
>>   exchanger) works well. I'm sure others will work just as well.
>>4. Dip teabag in destain solution with shaking. 
>
>>	The resin will clear the destain solution so that it doesn't
>>need changed. This allows destaining into a clear solution at a
>>faster rate. You can buy similar products commercially but why miss
>>out on all the teagbag fun !
>
>This seems awfully complicated and fiddly to me. I just throw in a piece
>of spongy foam (recycling of packaging materials!) and this soaks up the
>Coomassie just fine and bands are visible after a couple of hours. How 
>much faster is the teabag method?
>-- 

If foam is not available KimWipes work well too. Fold several together and
put in the corner of the destaining dish. Change when they go blue.
--
Allison Haggarty          ar229 at freenet.carleton.ca
                          mdah at musica.mcgill.ca




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